Journal: Journal of Translational Internal Medicine

Article Title: Effect of local anti-vascular endothelial growth factor therapy to prevent the formation of stenosis in outflow vein in arteriovenous fistula

doi: 10.2478/jtim-2021-0045

Figure Lengend Snippet: (A) Bioinformatics network of interaction among VEGF-A and its downstream-regulated molecules MMP2, TIMP1, TIMP2, and EphB4 by analyzing STRING PPI database. (B) MMP2, MMP9, TIMP1, and TIMP2 protein expression levels in the tissue of outflow veins in patients of different groups. MMP2, MMP9, TIMP1, and TIMP2 were higher in the AVF groups. (C) MMP2, MMP9, TIMP1, and TIMP2 in the four mice groups. The level of MMP9 expression was lower in the Avastin and LV-shRNA groups compared with the control-shRNA group on day 14 ( P < 0.05), and the level of MMP2 expression was lower in the Avastin group compared with the control-shRNA group ( P < 0.05). The expression of TIMP1 and TIMP2 in the outflow vein was significantly lower in the Avastin-treated group and LV-shRNA–transduced group compared with the control-shRNA group ( P < 0.05). Also, there was a substantial difference in TIMP2 expression between Avastin-treated and LV-shRNA groups ( P < 0.05). *Indicates a significant difference compared with the sham group ( P < 0.05). #Indicates a significant difference compared with the control-shRNA group ( P < 0.05). &indicates a significant difference compared with the LV-shRNA-VEGF group. Values are shown as means ± SD from three independent experiments. Representative images are shown from a total of four animals per group.

Article Snippet: After being transferred to PVDF membranes, the membranes were blocked with 5% skimmed milk and incubated with primary antibody: rabbit polyclonal antibody VEGF-A (Abcam, 1:600, ab1316), rabbit anti-EphB4 (Immunoway, 1:600, YT1587), rabbit anti-TIMP1 (Immunoway, 1:600, YT4658), rabbit anti-TIMP2 (Immunoway, 1:600, YT4659), and rabbit anti-caspase 3 (Immunoway, 1:600, ab2302), probing separately.

Techniques: Expressing, shRNA

Journal: Mediators of Inflammation

Article Title: Inhibitory Effect of Matrine on Blood-Brain Barrier Disruption for the Treatment of Experimental Autoimmune Encephalomyelitis

doi: 10.1155/2013/736085

Figure Lengend Snippet: Immunohistochemistry of MMP-2 and TIMP-2. At day 17 p.i., spinal cords were harvested after extensive perfusion. (a) MMP-2 and TIMP-2 expression was determined by immunohistochemistry. Magnification: ×40. (b) Quantitative analyses. Values represent mean ± SD ( n = 8 each group). △△ P < 0.01, compared to naive group; * P < 0.05 and ** P < 0.01, compared to the vehicle group; # P < 0.05, compared to the DEX group; ◊ P < 0.05, comparison between MAT-L and MAT-H groups.

Article Snippet: Immunohistochemistry was performed on these slices, using anti-rat antibodies for MMP-2 and TIMP-2 (all from Beijing TransGen Biotech Co.; Beijing, China).

Techniques: Immunohistochemistry, Expressing